Journal: BMC cancer

Article Title: Resolution of oncogene-induced senescence markers in HPV-infected cervical cancer tissue.

doi: 10.1186/s12885-025-13499-0

Figure Lengend Snippet: Fig. 1 Protein expression levels of Lamin B1 and Ki67in CC and CIN tissue samples. (A) Lamin B1. (B) Ki67. Protein expression was assessed by the percent age of DAP-positive cells within CIN (n = 32) and CC (n = 37) tissue samples. Statistical difference was calculated using Mann-Whitney test. *: pvalues < 0.05; ***: p values < 0. 001. Dots represent samples and line represent the median

Article Snippet: Following washing twice with phosphate-buffered saline (PBS), incubation with 3% hydrogen peroxide for 10 min (IHC only), and 5% bovine serum albumin (BSA) blocking, slides were incubated at room temperature for 2 h for Lamin B1 (1:600, Novus biologicals, Cat. No. NBP2-59783, clone 4001, USA), Ki67 (1:600, ab279653, Abcam, Cambridge, United Kingdom), and HPV-16 E6 + HPV-18 E6 antibodies (1/400, C1P5, sc-460, Santa Cruz Biotechnology, USA).

Techniques: Expressing, MANN-WHITNEY

Journal: BMC cancer

Article Title: Resolution of oncogene-induced senescence markers in HPV-infected cervical cancer tissue.

doi: 10.1186/s12885-025-13499-0

Figure Lengend Snippet: Fig. 2 Representative immunohistochemistry of Lamin-B1 and Ki67 staining in CC and CIN tissue samples. For both markers, protein expression was assessed by calculating the percentage of neoplastic cells exhibiting nuclear positivity. (A) and (B) demonstrate an increase in the median percentage of Lamin B1 positive tumor cells in CC samples compared to CIN samples, along with corresponding representative IHC images showing an increase in the percentage of positive neoplastic cells in a CC sample compared to a CIN sample, where positivity was confined to the neoplastic cells in the basal layers of the lesion. (C) and (D) demonstrate a more prominent increase in the median percentage of Ki67-positive tumor cells in CC samples compared to CIN samples, along with representative IHC images showing an increase in the percentage of cells exhibiting Ki67 nuclear positivity in a CC sample compared to a CIN sample showing limited basal positivity. IHC: immunohistochemistry

Article Snippet: Following washing twice with phosphate-buffered saline (PBS), incubation with 3% hydrogen peroxide for 10 min (IHC only), and 5% bovine serum albumin (BSA) blocking, slides were incubated at room temperature for 2 h for Lamin B1 (1:600, Novus biologicals, Cat. No. NBP2-59783, clone 4001, USA), Ki67 (1:600, ab279653, Abcam, Cambridge, United Kingdom), and HPV-16 E6 + HPV-18 E6 antibodies (1/400, C1P5, sc-460, Santa Cruz Biotechnology, USA).

Techniques: Immunohistochemistry, Staining, Expressing

Journal: BMC cancer

Article Title: Resolution of oncogene-induced senescence markers in HPV-infected cervical cancer tissue.

doi: 10.1186/s12885-025-13499-0

Figure Lengend Snippet: Fig. 3 Expression of Lamin B1 and Ki67 in co-localization with E6 in CIN tissue samples. Representative co-immunofluorescence staining fields showing few positively stained cells with Ki67 (red) and Lamin B1 (red) protein levels from E6 (green)-expressing cells in CIN tissue samples. Analysis of co-immu nofluorescence staining is shown in (Additional file 2, Figure S2)

Article Snippet: Following washing twice with phosphate-buffered saline (PBS), incubation with 3% hydrogen peroxide for 10 min (IHC only), and 5% bovine serum albumin (BSA) blocking, slides were incubated at room temperature for 2 h for Lamin B1 (1:600, Novus biologicals, Cat. No. NBP2-59783, clone 4001, USA), Ki67 (1:600, ab279653, Abcam, Cambridge, United Kingdom), and HPV-16 E6 + HPV-18 E6 antibodies (1/400, C1P5, sc-460, Santa Cruz Biotechnology, USA).

Techniques: Expressing, Immunofluorescence, Staining

Journal: BMC cancer

Article Title: Resolution of oncogene-induced senescence markers in HPV-infected cervical cancer tissue.

doi: 10.1186/s12885-025-13499-0

Figure Lengend Snippet: Fig. 4 Protein expression of E6 co-localized with Lamin B1 and Ki67 in CC tissue samples. Representative co-immunofluorescence staining fields showing wide positive staining of Ki67 (red) and Lamin B1 (red) protein levels from E6 (green)-expressing cells in CIN tissue samples Analysis of co-immunofluores cence staining is shown in (Additional file 2, Figure S2)

Article Snippet: Following washing twice with phosphate-buffered saline (PBS), incubation with 3% hydrogen peroxide for 10 min (IHC only), and 5% bovine serum albumin (BSA) blocking, slides were incubated at room temperature for 2 h for Lamin B1 (1:600, Novus biologicals, Cat. No. NBP2-59783, clone 4001, USA), Ki67 (1:600, ab279653, Abcam, Cambridge, United Kingdom), and HPV-16 E6 + HPV-18 E6 antibodies (1/400, C1P5, sc-460, Santa Cruz Biotechnology, USA).

Techniques: Expressing, Immunofluorescence, Staining

Journal: International Journal of Molecular Sciences

Article Title: Short Peptides Protect Fibroblast-Derived Induced Neurons from Age-Related Changes

doi: 10.3390/ijms252111363

Figure Lengend Snippet: List of primary antibodies for immunofluorescent staining live imaging analysis of functional activity of mitochondria and lysosomes.

Article Snippet: laminB1 , Novus (Chesterfield, MO, USA) , NBP2-59783 , 1/100.

Techniques: Staining, Imaging, Functional Assay, Activity Assay